Four Mistakes in Using Pipettes
Jul 07, 2022
The first mistake: When the pipette tip contains liquid, it is placed on the table.
Correction: Hang the pipette tip vertically on the pipette tip holder.
Analyses: The pipette tip contains liquid. After the pipette tip is placed on the table, the liquid may flow into the pipette tip body, which will affect the internal components of the pipette tip, and may cause serious damage and cross-contamination.
The second mistake: Use a large-capacity pipette tip to obtain liquids with small volumes.
Correction: The pipetting volume must be within the range provided by the pipette tip to meet the requirements of accuracy and precision.
Analyses: The existence of the air cushion is the main reason for the inaccuracy of the pipettor. The selection of the pipettor should be based on the selection principle of the maximum range and the liquid transfer volume closest to the target.
The third mistake: Absorb liquids with good volatility.
Correction: It is not recommended to use ordinary pipettors for transferring liquids with good volatility.
Analyses: If it is necessary to transfer a liquid with good volatility, the pipettor should be disassembled immediately after pipetting to allow the vapor to evaporate. At the same time, it is recommended to use an external piston pipettor.
The fourth mistake: The suction speed and discharge speed are too fast.
Correction: the liquid should be sucked and released slowly.
Analyses: For pipette tips with a volume of 1mL and above, it is easy to cause liquid to go upward. Discharging too quickly will increase the residual volume of the liquid.
Correction: Hang the pipette tip vertically on the pipette tip holder.
Analyses: The pipette tip contains liquid. After the pipette tip is placed on the table, the liquid may flow into the pipette tip body, which will affect the internal components of the pipette tip, and may cause serious damage and cross-contamination.
The second mistake: Use a large-capacity pipette tip to obtain liquids with small volumes.
Correction: The pipetting volume must be within the range provided by the pipette tip to meet the requirements of accuracy and precision.
Analyses: The existence of the air cushion is the main reason for the inaccuracy of the pipettor. The selection of the pipettor should be based on the selection principle of the maximum range and the liquid transfer volume closest to the target.
The third mistake: Absorb liquids with good volatility.
Correction: It is not recommended to use ordinary pipettors for transferring liquids with good volatility.
Analyses: If it is necessary to transfer a liquid with good volatility, the pipettor should be disassembled immediately after pipetting to allow the vapor to evaporate. At the same time, it is recommended to use an external piston pipettor.
The fourth mistake: The suction speed and discharge speed are too fast.
Correction: the liquid should be sucked and released slowly.
Analyses: For pipette tips with a volume of 1mL and above, it is easy to cause liquid to go upward. Discharging too quickly will increase the residual volume of the liquid.
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